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KMID : 0545120020120030476
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 3 p.476 ~ p.482
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Properties of Electron Carriers in the Process of Methanol Oxidation in a New Restricted Facultative Marine Methylotrophic Bacterium, Methylophagu sp. MP
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Koh, Moon Joo
Kim, Chun Sung/Kim, Yun A/Choi, Hack Sun/Cho, Eun Hee/Kim, Eung Bin/Kim, Young Min
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Abstract
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Methanol dehydrogenase (MDH), and c-type cytochromes from marine methanol-oxidizing bacterium, Methylophaga sp. MP, were purified and characterized. The native MDH had a molecular mass of 148 kDa and its isoelectric point was 5.5. Two c-type cytochromes, C_L and c_H, were found, and their isoelectric points were 3.4 and 8.0, respectively. The purified MDH had higher thermal stability than that of the other soil methylotrophic bacteria. The electron flow rate from MDH to cytochrome c_L was higher than that from MDH to cytochrome c_H, indicating that the physiological primary electron acceptor for MDH is cytochrome c_L. The electron transfer from MDH to phenazine ethosulfate (PES, artificial electron acceptor) in the two dye (PES/DCPIP)-linked assay system was not inhibited by NaCl, whereas the electron flow from MDH to cytochrome c_L, in the cytochrome/DCPIP-linked assay system was suppressed significantly by NaCl. Metal chelating agents such as EDTA showed the same effects on the MDH activity.
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